Semi Preparative Binary High Performance Liquid Chromatography HPLC Instrumentation

Semi Preparative Binary High Performance Liquid Chromatography HPLC Instrumentation

Description:

LC3000 I semi-preparative gradient system is designed for laboratory processing flow gradient elution conditions 50mL/min purification. The flow range suitable for inner diameter is 20 to 30mm chromatographic column, which can meet the single purified milligram to gram purification demand samples obtained, the purity and yield of the best. It is mainly used for separation and purification to gradient elution sample preparation. LC3000 I semi-preparative gradient system is also used in the preparation method validation and optimization. It will analyze the resulting liquid chromatography preparative chromatography conditions magnified to semi-preparative chromatography system; verify preparative chromatography conditions to further optimize the recommendation system.

Ordering Details:

Note:

* When the customers use the HPLC system for the first time, we suggest that solvent filter,

vacuum pump, ultrasonic cleaner, AC regulated power supply should be equipped.

Applications:

Purify products from micro gram level to gram level

1. High throughput screening active substance in drug development (phytomedicinem, microbial fermentation drug, synthetic drugs etc.)
2. Standard substance and reference substance preparation
3. Laboratory scale purification in research institute: from micro gram level to gram level
4. Purify impurities in drugs

Explore purification method for large scale purification

Technical parameter:

P3000 High-Pressure Infusion Pump

Technical Parameters

Flow Rate Range: ( 0 - 49.99 ) mL/min

Max Working Pressure: 25 MPa

Flow Accuracy: ± 2 % ( 5 mL/min)

Flow Repeatability: RSD ≤ 0.1 % ( 1 mL/min )

PSU: 220 V ± 22 V, 50 Hz, 120 W

Dimensions and Weights: 450 × 270 × 180 mm( L × W × H ) 12.8 kg

• UV3000 UV-VIS Detector

Wavelength Range: ( 190 - 700 ) nm

Light Source: D2 lamp (standard configuration), Tungsten lamp (optional available)

Band Width: 8 nm, Wavelength accuracy: ± 2nm, Wavelength repeatability:0.4nm

Baseline Noise: 1.5 × 10-5 AU/h

Baseline Drift: 4×10-4 AU/h

Dynamic: Velocity of flow 1mL/min; Methanol, wavelength 254 nm

Detection Cell Volume: 7 uL

PSU: 220 V ± 22 V, 50 Hz, 150 W

Dimensions and Weights: 450 × 270 × 180 mm ( L × W × H ) 10.4 kg

Competitive Advantage:

1. Solvent transmission double plunger reciprocating pump, double plunger suction, small pulsation;electronic compensation, velocity precision;
2. Slow start, stop, max / min pressure adjustable, user data is automatically stored.
3. High-voltage protection, it is automatically stopped over the set pressure value.
4. Dynamic hybrid, solvent mixed more fully connected, repeatability is better 1/16’’lines, convenient and practical.
5. Signal detection range 190-700nm wavelength detector, a wider range of applications.
6. GLP information features, easy to see the total lighting time, lighting times, the number of replacement lamps and product serial number and other device information.
7. 320 × 240 dot display large-screen LCD display, adjustable blue backlight, simple and generous.
8. Control Mode Independent control mode: LCD control panel buttons to set all operating parameters, making the operation more flexible.
9. Computer control modes: through the RS232 serial connection using CXTH workstation software control systems.
10. Mainly used in the separated from the mixture of purified monomer.
11. Versatility. It can be used for positive and negative phase solvent system.
12. Optional version and biological compatibility with commonly used versions are available to choose.